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Current life now…well, I just resigned on Monday. To rest…at home, from my worsen health condition, and all of the sudden, I am having fever now…>.<

Basically, life after resignation is relax and reckless…lol. I slept a lot. And straight away decide to go watch movie, no need wait for people, no need ask people to accompany, when and where to go, as I wish. 

By the way, this Saturday gonna go to Malacca with my friends…waiting forward…to be continued about my current life (not about visiting Malacca) later…

This few days was kinda attracted to posts in Facebook, especially from my lecturer, Dr. Choo. He posted: Tomorrow is the beginning of the new semester! Cepatnya! @@.  In one of the comments (comments were posted by his students and him), he said that, “jangan lupa pergi kelas besok ya. Pukul sebelas di Block B.” Then I commented on his status by saying that “Dr. Choo, can I attend the PoB class? I want to learn something new…XDXD”. He replied: Haha, you miss attending lectures ka? You’re always welcome to attend though 🙂 I consider this as no joke, although it might sound so, it is so nice to chat like that, especially with a lecturer, I think he recognized who am I, although just one period of time he has taught my class. Seriously, I feel like attend the class, because, I know that this is a good subject as a basic in understanding of Molecular Biology, however, my interest towards Molecular Biology is killed by Mr. Kam (for me, he is friendly, willing to help students, ya, as a criteria in communication, however, in terms of teaching, as a teacher, he DIDN’T contribute! He HAD NOT done his responsibility as a lecturer, to teach us properly. In a matter of fact, I HATE him!!! Sometimes, I think that, if I have interest in this subject, or if I score a good grade, if I learned more than I SHOULD be, I have more choices for my career. I know I couldn’t blame too much on him, but for me, the MAIN problem still because of him!!! I HATE you!!! When my good friends are talking about Molecular Biology thingy, I can’t join…mainly because I don’t really understand and the killed interest. So disappointed on this…

I know that Dr. Chew will be teaching Clinical Biochemistry in this semester, OMG, I wanted to be taught by her since last semester. The lucky thing is the previous lecturer didn’t kill my interest in Clinical Biochemistry. I guess because I like the subject very much. Haha. Is all about metabolism and pathology. Almost everytime I feel like disappointed because I can’t be taught by her on last semester, I know she is a VERY GOOD and EXPERIENCED lecturer. Big SIGH. All these make me even eager to attend lectures. XD

I MISS ATTENDING LECTURES!!!

Well, I guess, it has been 3-4 months, since I blogged here. Well, mainly is because I have no time to beautify the language I used for blogging, no time to describe everything (my style), maybe I am just lazy to log in here (not really possible), whatsoever. Now, I am back!!!

Firstly, to say that, I am graduated! Although results not that good, because of certain health conditions, I gone through it. My FYP grade, quite good, I guess it can help me in getting a job on lab-based? Hmm, sounds interesting.

Am back to hometown for some time since Tuesday night. Many things, especially clothes, still unpacked yet. Well, not enough space…and I really lazy to unpack all over while I think I might want to go to other place again for further study or work. Just don’t wish to stay at home (don’t ask me why). Unpacking some things since Wednesday, is tired, even not much, but due to the ached muscles on body. I am BORED TO DEATH, but, I am TIRED LIKE HELL.

Backward…backward… I still left some things at Kampar, no space in car to fit all of my things. Unload and load, arrange all the things, still left 4 more things: the chair, one SOFT mattress, and 2 more boxes, one super heavy and one not so heavy. Both of my soft bound and hard bound thesis are inside…>.< Now only I realized that I didn’t bring them back, my babies. LOL. I guess you all couldn’t imagine that I sat in such a small space in the car…scared some things might slip and knock on my head…=.= Totally feel like I was a sardin that time, squeezed inside the can. LUCKILY, I am thin and small enough to fit into the small space. If not, I might need to take the train to go back home. Tsk tsk. That means, I need to go to Kampar to collect my things. I have cause a little trouble to my friend who needs to get the things from my house and put in his house…paiseh, sorry, but thanks a lot for the help, appreciate it. I can take the chance to visit my friends if I am following. I may go to Ipoh on mid or end of June, so might stop by Kampar. Haha, I miss my friends very MUCH. XD

Well, has been a lot of people, especially my friends, even those who know me for not long asked me to change style. Maybe I “complained too much” about always being called “lengzai” when I go out. LOL… Normally those hawkers or shopkeeper or whoever, will call a girl as “lenglui” or a guy as “lengzai”. Guess what, I always being called as “lengzai” by those whoever…numbed and immuned for that…XD

Then another friend, who volunteered to be my fashion consultant. LOL. Don’t know whether is she joking or what. Ah, don’t care. Just feel surprised that, she said so. Because, other friends will just ask me to change style, but none of them will volunteer themselves. What I meant here is, because I don’t know how to choose so, my clothing normally is gender-neutral, plus I am quite an “airport”, a chinese will know what I meant here, short hair, that’s the reason why I always being misunderstood as a guy. Got some people even funny, they asked me that how should they called me as (those hawkers), my USUAL response is just smile. LOL. Speechless…~.~

Today, I tried a V-cut neck shirt. Wow, I can see the shoulder bone, first row of ribs…>.< plus, it is in slim cut form. Kinda girlish shirt…lolz. I plan of next Monday, wear the newly bought shirt, also V-cut, but is loose cutting with the skinny cut jeans. Hmm, what do you think? XD

Finally get the chance to do MBC, this is the value to determine after MIC is shown.

Yesterday tried all 9 compounds towards 2 kinds of bacteria: Staphylococcus aureus and Pseudomonas aeruginosa. Today, need to put the INT dye to observe the result. I didn’t expect that would have result. I hope I didn’t make any mistakes that showed positive result. MBC done by get 20 ul of suspension from the well to the Mueller-Hinton agar, then use cotton swab to evenly swab it to the space involved. Result will be observed on next Monday. Hopefully got positive result.  1 compound showed MIC value of 1 mg/ml and 500 ug/ml towards Staphylococcus aureus and 2 compounds, which 1 mg/ml for both of the compounds towards Pseudomonas aeruginosa.

MIC = Minimum Inhibition Concentration. This term is used in antimicrobial assay, by using 96-well microplate.

My planning for today is trying 9 compounds towards 2 types of bacteria, 2 microplates used.

Today, the air-conditioner suddenly dis-functioned, and I was wearing mask that time, to avoid the contamination caused by the breathing out.  On the Bunsen burner in the laminar hood. So, for the whole process of doing MIC, I felt very hot that time, sweating a lot that time. Is grateful that God granted me peace and patience. How would I sit so long there under so hot condition?! And I was paying full concentration. LOL.

Doing 18 rows of serial dilution, changing tips for every well, 8 x 18 = 144 times. Gosh. Fingers very tired and pain. 144 times is at least, you get me, including growth control, negative control, positive control and sterility control, and bacteria introduction to every well, change one tip for each row, at least 170 micropipette tips.

By the way, is getting familiar with MIC. I shall train myself more in pipetting skills. I must get myself skillful with that.

A big applause for myself today…ganbatte kudasai to myself.

Ok, morning, was waiting for my partner, so that we can go and get our glasswares. (Skip the waiting part, will only talk the important part). And yet, we still didn’t get the full set of the things we want yet. Damn!

Prepare to move all our glasswares and stuffs needed to the bench at BE (Biochemistry) FYP lab. Suddenly feel not used because things and reagents are just around us, now everything need to carry from the initial lab to the next. Just feel very not used to that…LOL

The biggest problem faced in antimicrobial assay is the solubility of the samples used in 1% DMSO. It is only 1%, is kinda hard. Never mind. Trial. Took 0.1mg of sample and prepare the 1% DMSO (by mixing 100ul of 100% DMSO and top up to 10mL with distilled water). Ya, not fully dissolved even shaked it vigorously. Then thought of using the sonicator, at last, the sample dissolved. Then boss suggested us to use 1 drop of 100% DMSO onto the sample then only mix it with the 1% DMSO. (The overall concentration of DMSO in antimicrobial assay cannot more than 6.25%) So, we try this suggestion. Ya, it worked.

Next week, gonna start the battle for antimicrobial assay…

As the title mentioned, antioxidant assay by microplate…it was began with a senior that saw me washed so many glasswares at a time…then the senior asked me about that. Then she taught me this method. Jump to conclusion first, it is very easy and very fast, LESS THINGS to wash…LOL.

I finished 50% of my FYP on Tuesday, which is 3 days ago. Then Wednesday onwards actually I no need to come to campus, because my partner hasn’t finish her part yet, so can’t proceed to antimicrobial assays. However, on Tuesday, the senior told me about that method, Wednesday should have trying that, but I forgot why I didn’t do that, however, tried that on yesterday and today. So far so good…^^ I only need to wash the micropipette tips and the microplate. The microplate no need to be thrown away because it is not dealing with microbes, so no need waste resources. Oh, by the way, my boss doesn’t know that I am doing this, so, don’t tell anyone ya…shhh…lolz.

What I need are only micropipette and the tips, 1.5 mL centrifuge tubes which can be just thrown away after using them, and a beaker for the used micropipette tips to be washed later. All this begin with prepare the master stock solution, then from the master stock, prepare the substock solution and also the DPPH solution. Then now is the turn of the role of microplate. Fill in the wells that involved with methanol, then with samples, the last is DPPH. Incubate for 15 minutes, then read it with microplate reader. Just as easy as that. And to finish the testing of one compound, needs only less than half an hour, compared to almost 45 minutes if used the not very appropriate method…!!! Yeah!!!

Tried it with the BHA, which is a standard antioxidant compound and 2 compounds that I wished to repeat the test (shown error in reading of absorbance). So far so good…^^ Happy with this…=)

On Friday morning, went to campus with a group of friends (I didn’t cycle to campus that day because rained before that, and since we were going to campus for the same purpose). We went there for the antimicrobial assay briefing by Dr. Sit.

He started to give us briefing. We all paid attention. Haha, he even noticed that I am not from biomedical science…lol. He really remembered who he taught? I also got asked him some questions and he explained to me in patience…^^

He explained very detail to us. Well, not to say we were not understand that time, is that we need TIME to digest and analyze. Hope everything is ok for us. (My friends’ co-supervisor is my boss, therefore…) Ms. Teo (my friends’ supervisor) knew that I will be doing the antimicrobial assay too, so asked me to listen to the briefing too, I am grateful for that…^^

Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus, 96-well microplate, antibiotic susceptibility test, disk diffusion, broth dilution, Mueller-Hinton agar, 625nm positive control, negative control, growth control, sterility control, etc. Wah…

Conclusion is I felt so fruitful today. Even though haven’t start to do it, I feel it is challenging and gonna be fun in learning this. Yeah!

Departed from Kampar to Penang at around 9:40am (before this had breakfast with friends), reached Penang around 11:30am.

Had lunch with buddies that joined the hiking and camping. Then, cars were 浩浩荡荡 went to the destination: Taman Negara Pulau Pinang. Unload things from cars…wah, like moving house…@@ so damn a lot of things…

Got a few members go to the camping site with our backpacks and belongings. I still hold my bag…because seems that I sensed something will happen. And one stick, this was very helpful for me…because my legs are fragile, in the sense of both of my ankles sprained before, I scared to walk down hill. Pace got slower when walked down the hill…

Started the hiking part. Got 2 groups. I was in the first group at first…talked and hiked. Wow, couldn’t they walk slower? In the end, my friend and me (I believed that my friend was waiting for me, thanks…^^) got lost when nearly arrive the beach. That time got 2 routes. Don’t know which route to follow, thats why waiting at the junction for the second hiking group… Finally reached Pantai Kerachut. Wow, the scenery is NICE!!! Snap, snap, snap photos, hehe.

Members that reached the destination by boat set the tents, while we rest and clean the gathering site. My shoes filled in a lot of small thingy, sand (a lot), got ants some more…lol. My friends said, sure the ant 难产而死…=.= Chair ball started, I don’t want get myself so tired or so dirty, so I didn’t really play. Rest…then around 6:30pm, had our dinner… most famous chicken rice in Penang!!! Wow, is very very nice. Since there was extra rice, I ate half of one pack after I finished the first pack…ehem. Then went to the beach where the ladies were sitting there. I also busy snap scenery photos there. Then Wee Khee let us sit in a row then he took our back view… those photos are awesome!!!

Card game at night. The card game called “Big 4”. The rules is like “chor dai di”, however, the biggest “di” can override by 3 cards of same number, any 3 cards, or 4 same cards can override 2 same cards. But, no 同花顺 or something similar that. By the way, is still very challenging. There is 3 groups of people playing, first, second and third group. First and second group consists of 3 people in each group, while third group consists of 4 people. Only the first people who first finish the cards in the FIRST group can gain one mark, the second one gains half mark, the last one no mark. Then the second person demoted to second group, so to the last person demoted to last group. This rule happens to every group, is that only you can only gain marks in FIRST group. Card games is temporarily hanged around 10-11pm, then most of them went for BBQ, left me and Lee Sien guarded the stuffs and belongings. We chatted and some of them brought what we wanted…thanks o…^^ The sausages I had very nice!!! Then went to bath…(seriously, me got dirt phobia, so called 洁癖, I really don’t like this feeling, but is ok la). After BBQ, most of them continue card games, another kind of card games. I chatted with Chin Ling. Then some solutions needed to be done. (if don’t understand this statement, then please don’t ask…seriously, DON’T ask)

Before sleep, went to the beach, Felicia told us to use leg to sweep the sand, and we can see the fluorescence phenomenon. Anyone can explain this? I think there is some mineral in the sand on beach that can fluorescence. Then clean legs and sleep in tent. Seriously, this is the first time I slept in tent. Ok, I think until 3am I also can’t sleep, around 4am, I think so. Then woke up around 7am. So? I slept less than 3 hours…=.= Before I slept, I asked Felicia: do you hear something? Got someone snoring…lolz. Ya, is Wee Khee… >.< Slept for such amount, still I looked energetic. Really something wrong. Continue card games…(really 一班赌鬼…lolz) This time, I managed to stay at first group a lot. Hahaha…^^

Packing…then walked in quite nervous mode on the bridge to the jetty…(I scared of high altitude…>.<) Group photo session…haha… Wow, I looked yeng in sunglasses, haha…(在暗爽和自恋…lolz) The view of the hills from sea also very nice. Took some photos.

Headed to lunch then splitted back home to rest. After back, I don’t feel any tiredness at all, which was weird. Until 2-3am only I feel a little sleepy.